Not really, but ACL usually stands for access control list so it’s telling you that the connection Mastodon is trying isn’t authenticated. Are you using the same tokens for AWS? That could be a difference.
See if you can find a log on your server or if there is one on the S3 side where you can find more details.
Then join a more general server. There are literally tons of small general servers. Join one where they’re friendly. If you want to look “under the hood” before joining there are apps like ice cubes that lets you look at the local feed of each server. That way you can see who’s posting.